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AlphaLISA Surefire Ultra Human and Mouse Total GSK3β Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total GSK3β assay is a sandwich immunoassay for quantitative detection of total GSK3β in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TGS3B-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TGS3B-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TGS3B-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TGS3B-A-HV
Unit Size: 100 Assay Points
  • Glycogen synthase kinase-3 beta (GSK3β) is active in numerous central intracellular signaling pathways and regulates important cellular processes, from proliferation and immune response to glucose regulation and apoptosis. GSK3 kinase phosphorylation inhibits the activity of downstream targets such as β-catenin. GSK3 is regulated by cellular localization and by protein complex formation in the insulin pathway. Insulin activates PI3K, which phosphorylates AKT and in turn, activated AKT phosphorylates GSK3α on Ser21 and GSK3β on Ser9, resulting in GSK3 inactivation and inducing activation of glycogen synthase by dephosphorylation. GSK3 is considered a versatile target for various disease research applications, including oncology, neurological disorders, and diabetes.

    The AlphaLISA SureFire Human and Mouse Total GSK3β Detection Kit is a sandwich immunoassay for the quantitative detection of total GSK3β in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Metabolic
    Neuroscience
    Oncology
    Unit Size
    100 Assay Points

Resources

1-3 of 3 Resources
Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...