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AlphaLISA SureFire Ultra Human Phospho-FOXM1 (Thr600) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-FOXM1 (Thr600) assay is a sandwich immunoassay for quantitative detection of phospho-FOXM1 (Thr600) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PFOXM1-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PFOXM1-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PFOXM1-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PFOXM1-A-HV
Unit Size: 100 Assay Points
  • Forkhead box protein M1 (FOXM1) is a transcription factor expressed throughout the cell cycle, rising in late G1-phase, reaching its apex in S-phase, and remaining there in G2/M and late M-phase. FOXM1 plays a significant role in multiple key cell cycle phases and is known as the master regulator of the cell cycle. The transcriptional network associated with FOXM1 regulates many processes, including stemness, cell survival, DNA damage repair, DNA replication, cell G1/S transition, cell cycle, cell proliferation, clonal formation, migration capacity, invasion, metastasis, and tumor initiation and progression. FOXM1 is found to be overexpressed in numerous malignancies such as breast, colorectal, hepatocellular, ovarian, lung, and pancreatic cancers.

    The AlphaLISA SureFire Human Phospho-FOXM1 (Thr600) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-FOXM1 (Thr600) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology
    Unit Size
    10,000 Assay Points

Resources

1-3 of 3 Resources
Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...