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AlphaLISA SureFire Ultra Human Phospho-FGFR3 (pan-Tyr) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-FGFR3 (pan-Tyr) assay is a sandwich immunoassay for quantitative detection of phospho-FGFR3 (pan-Tyr) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PFGFR3-B500
Unit Size: 500 Assay Points
Part Number: ALSU-PFGFR3-B10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PFGFR3-B50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PFGFR3-B-HV
Unit Size: 100 Assay Points
  • Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane Receptor Tyrosine Kinase consisting of one intracellular tyrosine kinase domain. Upon ligand binding, the receptor dimerizes, and transphosphorylation of tyrosine kinase domains occurs. Activation of downstream signaling occurs via FRS2, which functions as a key adaptor protein, resulting in MAPK and PI3K/AKT activation. Genetic alteration of FGFR3 leads to constitutive activation of this receptor and has significant consequences in carcinogenesis, involved in lymphomas, urothelial, hepatocellular, lung, breast, and colorectal cancers.

    The AlphaLISA SureFire Human Phospho-FGFR3 (pan-Tyr) Detection Kit is a sandwich immunoassay for the quantitative detection of Phospho-FGFR3 (pan-Tyr) in cellular lysates, using Alpha Technology.

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Assay Technology
    Automation Compatible
    AlphaLISA SureFire Ultra
    Detection Method
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Therapeutic Area
    Unit Size
    50,000 Assay Points


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This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Application Note
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Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...