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AlphaLISA Surefire Ultra Human and Mouse Total ERK1/2 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TERK-A50K
Unit Size: 50,000 assay points
Part Number: ALSU-TERK-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-TERK-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TERK-A500
Unit Size: 500 assay points
  • Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA®SureFire®Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA®SureFire®Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Automation Compatible
    Yes
    Assay Target
    ERK1/2
    Assay Target Class
    Protein
    Detection Method
    Alpha
    Format
    Microplates
    Therapeutic Area
    Cardiovascular
    Central Nervous System
    Neuroinflammation
    Assay Pathway
    MAPK
    Brand
    AlphaLISA SureFire Ultra
    Quantity in a Package Amount
    50000.0 Units
    Unit Size
    50,000 assay points

Resources

1-2 of 2 Resources
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx
Application Note Icon Image
Application Note
Protein extraction using the Omni Bead Ruptor Elite with detection of phosphorylation events using AlphaLISA no-wash assays
protein extraction, immunoassays, homogenizer