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Extra control lysate for the AlphaLISA® SureFire® Ultra™ Human Phospho-ErbB3 (Tyr1289) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-ErbB3 (Tyr1289) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-ErbB3 (Tyr1289) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, MCF7 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 1 mM pervanadate for 60 minutes and lysed in 5 mL of SureFire Ultra Lysis Buffer.
AlphaLISA SureFire Ultra