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AlphaLISA Surefire Ultra Total eIF4E Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total eIF4E assay. This control is already provided in the AlphaLISA SureFire Ultra Total eIF4E assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total eIF4E Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate HEK293 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 200 ng/mL EGF for 30 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis Buffer.
Specifications
| Application |
Protein Detection
|
|---|---|
| Assay Technology |
Alpha
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Unit Size |
1 vial
|
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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