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AlphaLISA SureFire Ultra Human Phospho-Cyclin D1 (Thr286) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-Cyclin D1 (Thr286) assay is a sandwich immunoassay for quantitative detection of phospho-cyclin D1 (Thr286) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PCYCD1-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PCYCD1-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PCYCD1-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PCYCD1-A-HV
Unit Size: 100 Assay Points
  • Cyclin D1 is encoded by CCND1 and is a member of the highly conserved cyclin family, which includes Cyclin D2 and Cyclin D3. Cyclin family members are important regulators of cell cycle progression and act as mediatiors of growth factor dependent G1 to S phase progression by playing the role of allosteric regulators of CDK4/6. Cyclin D1 is an oncogene and is found to be overexpressed in many human cancers. Abnormal levels of Cyclin D1 result in dysregulated CDK4/6 activity and leads to tumorigenesis.

    The AlphaLISA SureFire Human Phospho-Cyclin D1 (Thr286) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin D1 (Thr286) in cellular lysates, using Alpha Technology.

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    Assay Target Class
    Assay Technology
    Automation Compatible
    AlphaLISA SureFire Ultra
    Detection Method
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Therapeutic Area
    Unit Size
    500 Assay Points


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Application Note
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