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AlphaLISA Surefire Ultra Human Total Aurora B Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Total Aurora B assay. This control is already provided in the AlphaLISA SureFire Ultra Total Aurora B assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TAURB-A-L
Part Number: ALSU-TAURB-A-L
  • This control lysate is intended for use with the AlphaLISA SureFire UltraHuman Total Aurora B Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in 10% FBS containing medium, then treated with 50 nM Calyculin A and 2 μM PMA for 90 minutes at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Therapeutic Area
    Oncology
    Unit Size
    1 Vial

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...