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AlphaLISA Mouse IL-18 Detection Kit, 100 Assay Points

The AlphaLISA® IL-18 Detection Kit is designed for the quantitative determination of mouse IL-18 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3189HV
Unit Size: 100 Assay Points
Part Number: AL3189C
Unit Size: 500 Assay Points
Part Number: AL3189F
Unit Size: 5,000 Assay Points
  • Interleukin-18 (IL-18), also known as interferon-gamma inducing factor, is a proinflammatory cytokine of the IL-1 family that is primarily secreted by macrophages. IL-18’s biological activity resembles that of IL-12, with the ability to enhance NK cell activity and induce IFNγ secretion by PMBC. A key role of IL-18 is its ability to eliminate VEGF from the eye to protect the retina. In combination with anti-VEGF antibodies, IL-18 is utilized to treat age-related macular degeneration (AMD). IL-18 dysregulation is also linked to severe inflammatory reactions.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Mouse
    Unit Size
    100 Assay Points