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Lentivirus for cell therapy

Lentiviruses are a subtype of retroviruses which have a single stranded RNA genome that encode for three major structural genes: gag, pol, and env.

Lentiviral vectors contain only the viral cis-regulatory elements necessary for genome integration, transcription, and packaging of the vector RNA. All viral protein coding regions are removed providing space for insertion of a foreign DNA sequence. Separating the lentiviral genome onto several plasmids reduced the risk of generating replication competent lentiviruses

In contrast with classical retroviruses, lentiviral vectors have the ability to transduce both dividing and non-dividing cells which are used as general tools for basic research and translational applications.


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