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Immunoassays Solutions

Simplify your immunoassays with a single source solution

In today's fast-paced research environment, having the right tools can significantly improve your workflow. Revvity delivers renowned, cutting-edge immunoassay solutions tailored to your needs and budget. Our connected ecosystem includes advanced assay technologies, powerful instrumentation, and expert support.

From traditional ELISAs to next-gen no-wash and flow-based methods, our solution adapts to your workflow - boosting efficiency, speeding up results, and helping you push the boundaries of discovery.


For research use only. Not for use in diagnostic procedures.

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Immunoassays solutions

Wash-based immunoassays

wash-based - elisa

ELISA

Plate-based, colorimetric immunoassay.

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Wash-based immunoassays - LEGENDplex

LEGENDplex™

Fluorescence bead-based assay for simultaneous quantification of up to 14 analytes.

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wash-based - ELISpot

ELISpot

High-sensitivity, cell- based assay for single-cell level analyte detection.

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wash-based - DELFIA

DELFIA™

Stable, time-resolved fluorescent signal to improve assay dynamic range.

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No-wash immunoassays

no-wash - alpha

Alpha

Versatile bead-based, no-wash, luminescent technology.

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no-wash - HTRF

HTRF™

No-wash technology, gold reference for time-resolved FRET.

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no-wash - LANCE

LANCE™

No-wash proximity assay technology that combines the benefits of TR with FRET.

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Immunoassays workflow

Sample prep

sample prep - omni

Sample Homogenization

Leverages the power of mechanical shearing to maximize tissue homogenization and cell lysis.

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Liquid handling

liquid handling - fontus

Liquid Handling

Liquid handling solutions for over 40 years to help labs accelerate their science.

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Reagents

Immunoassays

Immunoassays

Discover our range of immunoassay technologies that enable the selection of optimal immunoassay platforms for your requirements.

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Custom Assay Development Services

Over 30 years of expertise crafting custom assays precisely tailored to your unique research needs.

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Microplates

microplates

Microplates

Our microplates have been engineered to deliver the highest quality data for various applications.

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Read-out

plate reader - nexus

Multimode Plate Readers

Configurable multimode plate readers equipped with the latest technologies for labs today.

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Image Cytometry

Fast, accurate and efficient system to assess cell health and viability.

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Mini ELISA Plate Reader™

Delivers fast, compact ELISA analysis with simple plug-and-play USB connectivity - now 50% off.

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Lab automation

lab automation

Lab Automation

Custom integrated workstations, providing innovative automation solutions to simplify lab workflow.

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Software

signals

Signals Software

Software solutions that leverage the latest technologies to help simplify science and accelerate innovation.

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Explore immunoassays applications

Protein quantification

Determine the parameter most critical to your research to find the optimal immunoassay technology.

Technology
  • Absorbance, Luminescence, Colorimetric - ELISA
  • TRF, TR-FRET, Advanced Fluorescence – HTRF
  • Advanced Luminescence - Alpha
  • Fluorescence intensity, Standard Fluorescence – LEGENDplex*
Throughput
  • No automation - ELISA, HTRF, Alpha, LEGENDplex*
  • Automation - HTRF, Alpha
Time to results
  • Wash steps are fine, no major interference with my daily operations and/or needed to remove non-bound material – ELISA, LEGENDplex*
  • Need no-wash protocols to save time for daily operations - HTRF, Alpha
  • No preference - ELISA, LEGENDplex*, HTRF, Alpha
Sample size
  • < 1,000 data points/week - ELISA, HTRF, Alpha
  • > 1,000 data points/week - HTRF, Alpha, LEGENDplex*
Sample type
  • Cell lysate - ELISA, HTRF, Alpha, LEGENDplex*
  • Supernatant (+10% FCS) - ELISA, HTRF, LEGENDplex*, Alpha
  • Urine – ELISA, HTRF, Alpha, LEGENDplex*
  • Serum - ELISA, Alpha, LEGENDplex*
  • Plasma - ELISA, Alpha, LEGENDplex*
Sample volume
  • 5 to 20µl - HTRF, Alpha
  • 20 to 50µl - ELISA, HTRF, Alpha, LEGENDplex*
  • No limitations - ELISA, HTRF, Alpha, LEGENDplex*
Concentration range
  • Up to 2 logs (<100) - ELISA, HTRF, Alpha
  • More than 2 logs (>100 fold) & may vary from sample to sample - HTRF, Alpha, LEGENDplex*


*Requires flow cytometer capable of reading APC and PE or, in the case of instruments without a red laser, PE and PerCP.

Second messenger detection

Determine the parameter most critical to your research to find the optimal immunoassay technology.

Technology
  • TRF, TR-FRET, Advanced Fluorescence - HTRF
  • Advanced Luminescence - Alpha
Throughput
  • No automation - HTRF, Alpha
  • Automation - HTRF, Alpha
Time to results
  • Need no-wash protocols to save time for daily operations - HTRF, Alpha
Sample size
  • < 1,000 data points/week - HTRF, Alpha
  • > 1,000 data points/week - HTRF, Alpha
Sample type
  • Cell lysate - HTRF, Alpha
  • Primary cells - HTRF, Alpha
  • Tissue lysates - HTRF, Alpha
  • Membrane preparations - HTRF
  • iPSC-derived cells - HTRF, Alpha
  • 3D models - HTRF, Alpha
Sample volume
  • 5 to 20µl - HTRF, Alpha
  • 20 to 50µl - HTRF, Alpha
  • No limitations - HTRF, Alpha
Concentration range
  • Up to 2 logs (<100) - HTRF, Alpha
  • More than 2 logs (>100 fold) & may vary from sample to sample - HTRF, Alpha

Protein-protein interaction

Determine the parameter most critical to your research to find the optimal immunoassay technology.

Technology
  • TRF, TR-FRET, Advanced Fluorescence - HTRF
  • Advanced Luminescence - Alpha
Throughput
  • No automation - HTRF, Alpha
  • Automation - HTRF, Alpha
Time to results
  • Need no-wash protocols to save time for daily operations - HTRF, Alpha
Sample size
  • < 1,000 data points/week - HTRF, Alpha
  • > 1,000 data points/week - HTRF, Alpha
Sample type
  • Buffer - HTRF, Alpha
  • Cell lysate - Alpha
Sample volume
  • 5 to 20µl - HTRF, Alpha
  • 20 to 50µl - HTRF, Alpha
  • No limitations - HTRF, Alpha

Cell signaling

Determine the parameter most critical to your research to find the optimal immunoassay technology.

Technology
  • TRF, TR-FRET - HTRF
  • Advanced Luminescence - Alpha
Throughput
  • No automation - HTRF, Alpha
  • Automation - HTRF, Alpha
Time to results
  • Need no-wash protocols to save time for daily operations - HTRF, Alpha
Sample size
  • < 1,000 data points/week - HTRF, Alpha
  • > 1,000 data points/week - HTRF, Alpha
Sample type
  • Cell lysate - HTRF, Alpha
  • Primary cells - HTRF, Alpha
  • Tissue Lysates - HTRF, Alpha
  • iPSC-derived cells - HTRF, Alpha
  • 3D Models - HTRF, Alpha
Sample volume
  • 5 to 20µl - HTRF, Alpha
  • 20 to 50µl - HTRF, Alpha
  • No limitations - HTRF, Alpha

Receptor ligand binding

Determine the parameter most critical to your research to find the optimal immunoassay technology.

Technology
  • TRF, TR-FRET, Advanced Fluorescence - HTRF
  • Advanced Luminescence - Alpha
Throughput
  • No automation - HTRF, Alpha
  • Automation - HTRF, Alpha
Time to results
  • Need no-wash protocols to save time for daily operations - HTRF, Alpha
Sample size
  • < 1,000 data points/week - HTRF, Alpha
  • > 1,000 data points/week - HTRF, Alpha
Sample type
  • Buffer - HTRF, Alpha
  • Cells - HTRF
Sample volume
  • 5 to 20µl - HTRF, Alpha
  • 20 to 50µl - HTRF, Alpha
  • No limitations - HTRF, Alpha
Concentration range
  • Up to 2 logs (<100) - HTRF, Alpha
  • More than 2 logs (>100 fold) & may vary from sample to sample - HTRF, Alpha

Explore modalities

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Featured combinations

LEGENDplex LEGENDplex
Omni Bead Ruptor Elite Bead Mill Homogenizer Omni bead mill homogenizers

Quantification of cytokines from tissue homogenate

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Quantification of cytokines from tissue homogenate

Resources

Webinar: Streamlining the quantification of immune mediators in diverse tissues
Webinar

Streamlining the quantification of immune mediators in diverse tissues

The webinar showcases a workflow using the Omni Bead Ruptor Elite™ homogenizer for tissue lysate preparation and the LEGENDplex™ panel for simultaneously measuring 13 key cytokine release syndrome (CRS) biomarkers. This approach provides insights into CRS mechanisms and supports immunotherapy development.

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Application note: Rapid inflammatory cytokine quantification from serum and skin
Application note

Rapid inflammatory cytokine quantification from serum and skin

This application note compares protein extraction methods for skin samples and presents data on 13 inflammatory cytokines measured in both serum and skin from an LPS-induced CRS mouse model using LEGENDplex™ panels.

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Application note: Quantification of inflammatory cytokines from spleen and liver tissue
Application note

Quantification of inflammatory cytokines from spleen and liver tissue

Cytokine Release Syndrome (CRS) research focuses on preventing tissue damage. Our workflow uses LEGENDplex™ panels and the Omni Bead Ruptor Elite to prepare tissue lysates and quantify 13 CRS biomarkers. Results confirm bead mill homogenization effectively disrupts tissues for cytokine measurement in inflammatory process research.

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AlphaLISA AlphaLISA
Omni Omni bead mill homogenizers
Envision EnVision

Sample prep - protein extraction & phosphorylation detection

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Sample prep - protein extraction & phosphorylation detection

Resources

Application note: Protein extraction and phosphorylation detection in mouse kidneys
Application note

Protein extraction and phosphorylation detection in mouse kidneys

Bead milling provides an efficient tissue homogenization alternative without harsh chemicals or cryogenic processing. This technique was validated using 5-FU treated mice, enabling analysis of phospho- and total-ERK biomarkers linked to kidney disease in protein lysates.

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HTRF technology HTRF
Omni Bead Ruptor Elite Bead Mill Homogenizer Omni bead mill homogenizers

Analyzing brain samples with HTRF

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Analyzing brain samples with HTRF

Resources

Technical note: Best practices for analyzing brain samples in neurosciences
Technical note

Protocol for brain sample analysis with HTRF

This technical note provides insider tips for maximizing brain sample analysis through effective lysis, accurate protein quantification, and optimized HTRF assay execution.

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No wash immunoassays No-wash immunoassays
Envision Nexus EnVision Nexus Multimode Plate Reader

High throughput and ultra-sensitive detection

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High throughput and ultra-sensitive detection

Resources

Technical note: TRF assay performance of the EnVision Nexus Multimode Plate Reader
Technical note

Superior TRF assay performance

The EnVision Nexus™ multimode plate reader excels in TRF assays with HTRF™ and LANCE™ technologies, offering enhanced performance for high-throughput screening.

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Technical note: Ambient temperature for exceptional Alpha performance
Technical note

Ambient temperature for exceptional Alpha performance

The EnVision Nexus™ plate reader enhances AlphaLISA™ and AlphaScreen™ no-wash assays with temperature-controlled precision for superior protein interaction studies.

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No wash immunoassays No-wash immunoassays
Liquid handling Liquid handling
Envision Nexus EnVision Nexus Multimode Plate Reader
Lab Automation/Signals Workflow automation

Full Automation for High-Throughput Screening

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Full Automation for High-Throughput Screening

Resources

ebook: Multimode plate readers for high-throughput screening
ebook

Multimode plate readers for high-throughput screening

Revvity accelerates lab workflows with integrated instruments, reagents & software. Our automation-ready multimode plate readers, paired with industry-leading assays, advanced liquid handling, and automation workstations can help researchers deliver therapies to market faster.

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Featured resources

Whitepaper

Reimagining immunoassay workflows: The integrated future of discovery

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Flyer

Simplify your immunoassays with an integrated resource.

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Infographic

Immunoassays workflow solution

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Literature - Publication Review

Beyond GLP-1: Emerging targets and mechanisms for next-generation metabolic therapeutics.

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Brochure

Multimode plate readers: At the forefront of high-throughput screening

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