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LANCE Ultra Human Fibronectin Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF1351C
Unit Size:
Part Number: TRF1351M
Unit Size:
  • The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    • No-wash steps, no separation steps
    • TR-FRET technology
    • Sensitive detection
    • High reproducibility
    • Faster time-to-results
    • Easy automation
    • 96-well, 384-well, and 1536-well formats

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

    Fibronectin is a large glycoprotein of the extracellular matrix that exists in two forms: insoluble (cellular) and as a soluble plasma protein. The primary functions of fibronectin are its involvement in cell adhesion, growth, migration, and differentiation. Fibronectin is known to contribute to the wound healing process where it will accumulate at the site of injury to help form blood clots and aid in the development of new tissues. Fibronectin levels in plasma have been identified to increase significantly after major traumas. Further, Fibronectin levels have been observed to be significantly altered in disease states of certain types of cancer and also diabetes mellitus.

  • Application
    Protein Analysis & Detection
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity
    1
    Therapeutic Area
    Oncology