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LANCE Ultra Human ANGPT-1 Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human ANGPT1 Detection Kit is designed for detection and quantitation of human angiopoietin 1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF3014C
Unit Size:
Part Number: TRF3014M
Unit Size:
  • The LANCE®Ultra Human ANGPT1 Detection Kit is designed for detection and quantitation of human angiopoietin 1 (ANGPT1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    • No-wash steps, no separation steps
    • TR-FRET technology
    • Sensitive detection
    • High reproducibility
    • Faster time-to-results
    • Easy automation
    • 96-well, 384-well, and 1536-well formats

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

    Angiopoietin belongs to a family of vascular growth factors. There are several angiopoietins: ANGPT1, ANGPT2, ANGPT4 now identified, and a number of closely related proteins: ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL5, ANGPTL6, and ANGPTL7. Angiopoietin 1(Ang-1; also ANGPT1) is a 70 kDa glycoprotein which contains 498 amino acids, including a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. It plays a critical role in vessel maturation, adhesion, migration, and survival.

  • Application
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Format
    Microplates
    Therapeutic Area
    Oncology