Skip to main content

HTRF Mouse IL-2 Detection Kit, 10,000 Assay Points

The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62MIL02PEG
Unit Size: 500 Assay Points
Part Number: 62MIL02PEH
Unit Size: 10,000 Assay Points
  • IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes differentiation of T cells into effector or memory T cells, thus helping body fight off infection. IL2 is also a key player in immuno-oncology since it is main promoter of differentiation of T cells into Treg (regulatory T cells).

    Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

  • Assay Points
    10000
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Neuroscience
    Oncology & Inflammation
    Unit Size
    10,000 Assay Points
  • Assay principle

    Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

     

    1cytokines-how-it-works-assay-principle-human-il13-62hil13peg-62hil13peh.svg

     

    Assay data analysis

    The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

    To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, 
    Revvity also worked with Myassays.com to help you in your data analysis., you’ll be able to access a free online software to run your IL2 analysis.

     

  • Technical specifications of mouse IL2 kit

     

    Sample size 16 µL
    Final assay volume 20 µL
    Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
    LOD &LOQ (in Diluent) 12 pg/mL &35 pg/mL
    Range 35 – 2,560 pg/mL
    Time to result Overnight at RT
    Species Mouse only

     

     

  • Intra and inter assay

    Intra-assay (n=24)

     

    Sample Mean [IL2] (pg/mL) CV
    1 100 9%
    2 900 3%
    3 1800 7%
      Mean CV 6%

     

    Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

     

    Inter-assay (n=4)

     

    Sample [IL2] (pg/mL) Mean (delta R) CV
    1 80 329 2%
    2 320 1461 3%
    3 1280 5980 8%
        Mean CV 4%

     

    Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

     

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.