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HTRF Human FMRP Detection Kit, 500 Assay Points

The Human FMRP kit is designed for the rapid detection of human FMRP in cell lysates.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 63ADK038PEG
Unit Size: 500 Assay Points
List price: USD 1,039.00
Your price:
USD 1,039.00
USD 1,039.00 /each
Part number: 63ADK038PEH
Unit Size: 10,000 Assay Points
List price: USD 11,750.00
Your price:
USD 0.00
USD 11,750.00 /each

Overview

FMRP (Fragile X Mental Retardation Protein) is a brain protein known to play an important role in normal cognitive development and female reproductive function. fmr1 gene silencing has been correlated to fragile X syndrome onset triggering severe learning deficits or mental retardation. Mutations of the fmr1 gene can also be associated to intellectual disability, premature ovarian failure, autism, Parkinson's disease, developmental delays, and other cognitive deficits. The human FMRP kit enables the fast monitoring of FMRP produced by cells.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Therapeutic Area
Neuroscience
Rare Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

Human FMRP is measured using a sandwich immunoassay involving two specific anti-FMRP antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the FMRP present in the sample.

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Assay protocol
The human FMRP assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human FMRP. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
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Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, Manuals and more

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