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AlphaScreen Histidine (Nickel Chelate) Detection Kit, 500 Assay Points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 6760619C
Unit Size: 500 Assay Points
List price: USD 282.18
Your price:
USD 282.18
USD 282.18 /each
Part number: 6760619M
Unit Size: 10,000 Assay Points
List price: USD 4,528.00
Your price:
USD 0.00
USD 4,528.00 /each
Part number: 6760619R
Unit Size: 50,000 Assay Points
List price: USD 21,720.00
Your price:
USD 0.00
USD 21,720.00 /each

Overview

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • His-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.

Specifications

Antibody Conjugates
Streptavidin/Nickel chelate
Application
Protein-protein interactions
Assay Points
500
Automation Compatible
Yes
Brand
AlphaScreen
Species
Human
Unit Size
500 Assay Points

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Resources

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Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

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