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AlphaScreen Histidine (Nickel Chelate) Detection Kit, 500 Assay Points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6760619C
Unit Size:
Part Number: 6760619M
Unit Size:
Part Number: 6760619R
Unit Size:
  • This bead kit can be used for many applications, including:

    • Protein-protein interaction assays
    • Protein-peptide interaction assays
    • His-tagged protein detection and quantitation
    • Protein expression
    • Binding studies

    In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.

  • Antibody Conjugates
    Streptavidin/Nickel chelate
    Application
    Protein-protein interactions
    Assay Points
    500
    Automation Compatible
    Yes
    Brand
    AlphaScreen
    Species
    Human

Resources

1-1 of 1 Resources
Guide
Alpha Protein-Protein Interaction Quick Start Guide
alphalisa, protein, assay, immunoassay