Skip to main content

AlphaScreen Nickel Chelate Donor Beads, 5 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AS101M
Unit Size: 5 mg
Part Number: AS101R
Unit Size: 25 mg
Part Number: AS101D
Unit Size: 1 mg
  • Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

    • Protein-protein interaction assays
    • Protein detection assays
    • Enzymatic assays

    In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

  • Automation Compatible
    Yes
    Application
    Protein-protein interactions
    Bead Type or Core Bead Type
    Alpha Donor
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Antibody Conjugates
    Nickel chelate
    Brand
    AlphaLISA
    Unit Size
    5 mg

Resources

1-6 of 6 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions
DNA-protein, DNA:protein, binding, interaction, protein:DNA, protein-DNA, AlphaLISA, EMSA
Guide book
Alpha Protein-Protein Interaction Quick Start Guide
AlphaLISA, protein, assay, immunoassay
Guide book
Alpha Protein-Protein Interaction Quick Start Guide
AlphaLISA, protein, assay, immunoassay
Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.
AlphaLISA, toolbox, ADCC, biochemical, hIgG, assays
Guide book
ELISA to AlphaLISA Immunoassay Conversion Guide
ELISA conversion, AlphaLISA, long guide
Guide book
ELISA to AlphaLISA Immunoassay Conversion Guide
ELISA conversion, AlphaLISA, long guide