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AlphaScreen Nickel Chelate Donor Beads, 25 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AS101D
Unit Size:
Part Number: AS101M
Unit Size:
Part Number: AS101R
Unit Size:
  • Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

    • Protein-protein interaction assays
    • Protein detection assays
    • Enzymatic assays

    In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

  • Antibody Conjugates
    Nickel chelate
    Protein-protein interactions
    Automation Compatible
    Bead Type or Core Bead Type
    Alpha Donor
    Detection Method
    Experimental Type
    In vitro


1-4 of 4 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions
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Alpha Protein-Protein Interaction Quick Start Guide
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Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.
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ELISA to AlphaLISA Immunoassay Conversion Guide
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