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AlphaScreen cAMP Detection Kit, 10,000 Assay Points

AlphaScreen no-wash assay kit for quantitative detection of cAMP.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6760635D
Unit Size: 1,000 Assay Points
Part Number: 6760635M
Unit Size: 10,000 Assay Points
Part Number: 6760635R
Unit Size: 50,000 Assay Points
  • AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.

    This kit contains enough reagents to run 1,000 wells using a 25 µL reaction volume. Protocol can be found on the Certificate of Analysis.

    This product replaces 6760625M.

  • Application
    cAMP
    Assay Points
    10000
    Automation Compatible
    Yes
    Brand
    AlphaScreen
    Quantity in a Package Amount
    1.0 per pack
    Second Messenger Release
    cAMP
    Therapeutic Area
    Metabolic
    Unit Size
    10,000 Assay Points

Resources

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Application Note Icon Image
Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit

This note presents data showing performance of an AlphaScreen cAMP assay in a 384-well plate. The assay was evaluated for its...