Skip to main content
Menu
US

AlphaPlex 545 (Terbium) Anti-unmodified H3K4 Acceptor Beads, 250 µg

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AP119TB-C
Unit Size: 250 µg
List price: USD 2,234.00
Your price:
USD 2,234.00
USD 2,234.00 /each
Part number: AP119TB-M
Unit Size: 5 mg
List price: USD 11,540.00
Your price:
USD 0.00
USD 11,540.00 /each
Part number: AP119TB-R
Unit Size: 25 mg
List price: USD 40,710.00
Your price:
USD 0.00
USD 40,710.00 /each

Overview

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

Alpha technology is a no-wash homogeneous proximity immunoassay that includes Alpha Donor and AlphaPlex-545 Acceptor beads to quantitate activity on histone, peptide, or protein substrates. In vitro modification of substrate is performed by incubating enzyme with appropriate cofactor and histone substrate. Following the enzymatic reaction, methylation or acetylation is monitored using specific antibody-conjugated AlphaPlex Acceptor beads. Following enzymatic reaction,streptavidin-coated Donor beads are added to capture the substrate, the beads are brought into close proximity in the absence of epigenetic mark on Histone H3 (Lysine 4) on the target substrate. The Donor beads are excited by a laser at 680 nm to release singlet oxygen. The singlet oxygen causes a reaction in the Acceptor bead and light emission is produced at 545 nm. This emission is proportional to the amount of unmodified / non-methylated / non-acetylated substrate present.

AlphaPlex-545 Acceptor beads are intended for use in multiplexing Alpha assays, in conjunction with Alpha Donor beads and AlphaLISA® Acceptor beads.

  • 250 µg of Acceptor beads is sufficient to run ~500 wells using a 50 µL reaction volume.
  • 5 mg of Acceptor beads is sufficient to run ~10,000 wells using a 50 µL reaction volume.
  • 25 mg of Acceptor beads is sufficient to run ~25,000-50,000 wells using a 50 µL reaction volume.

Specifications

Antibody Conjugates
Anti-H3K4
Application
Epigenetics
Automation Compatible
Yes
Bead Type or Material
AlphaPlex Terbium (545) Acceptor
Brand
AlphaPlex
Detection Method
Alpha
Format
Microplates
Molecular Modification
Unmodified
Shipping Conditions
Shipped in Blue Ice
Unit Size
250 µg

Video gallery

Resources

1-3 of 3 Resources
Guide Icon
Guide
AlphaPlex quick start guide

This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...

Whitepaper Icon
Whitepaper
Epigenetic modifications and how they relate to cancer development and therapy

Epigenetic modifications in cancer

The field of epigenetics has revealed revolutionary methods for understanding heredity...

Flyer Icon
Flyer
Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon