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AlphaLISA Human VCAM-1 Detection Kit, 5,000 Assay Points

The AlphaLISA® Human VCAM-1 Detection Kit is designed for detection and quantitation of human Vascular Intracellular Adhesion Molecule 1 (VCAM-1) in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL338C
Unit Size: 500 assay points
Part Number: AL338F
Unit Size: 5,000 Assay Points
Part Number: AL338HV
Unit Size: 100 Assay Points
  • Formats:

    • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Vascular Cell Adhesion Molecule-1 (VCAM-1) is a transmembrane glycoprotein with a molecular weight of 110 kDa. VCAM-1 contains six to seven immunoglobulin domains and is expressed on vascular endothelial cells of both small and large blood vessels after stimulation via cytokines (i.e. TNFα, IL-4). It mediates immune system response by recruiting additional immune cells to sites of inflammation. After VCAM-1 binds to either Very Late Antigen 4 (VLA-4) or Integrin receptor α4β7 a proteolytic ectodomain shedding process triggers the release of a soluble form of VCAM-1 (sVCAM-1). This soluble form can be utilized as an inflammatory biomarker. Serum levels of sVCAM-1 have been found to be altered in patients with cancer, diabetes, atherosclerosis, rheumatoid arthritis, and other autoimmune diseases. The AlphaLISA kit presented here detects sVCAM-1 in serum, plasma, and cell culture media.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target
    VCAM-1
    Assay Target Class
    Biomarker
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Oncology
    Unit Size
    5,000 Assay Points