The AlphaLISA™ SARS-CoV2 Nucleocapsid Detection Kit is a sandwich immunoassay for quantitative detection of SARS-CoV2 Nucleocapsid in cell lysates and cell supernatants using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 70 - 100,000 pg/mL |
Limit of Detection | 70 pg/mL |
Limit of Quantification | 231 pg/mL |
Sample Volume | 5 µL |
The AlphaLISA™ SARS-CoV2 Nucleocapsid Detection Kit is a sandwich immunoassay for quantitative detection of SARS-CoV2 Nucleocapsid in cell lysates and cell supernatants using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The SARS-CoV-2 nucleocapsid (N) protein is a multivalent RNA-binding protein. It plays a critical role in forming helical ribonucleoproteins during packaging the RNA genome, regulating viral RNA synthesis during replication, transcription, and modulating infected cell metabolism. The N protein is highly expressed within infected cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
70 - 100,000 pg/mL
|
Limit of Detection |
70 pg/mL
|
Limit of Quantification |
231 pg/mL
|
Product Group |
Kit
|
Sample Volume |
5 µL
|
Shipping Conditions |
Dual Temperature
|
Target |
SARS-CoV2 Nucleocapsid
|
Target Class |
Viral Particles
|
Technology |
Alpha
|
Therapeutic Area |
Virology
|
Unit Size |
500 Assay Points
|
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