AlphaLISA Mouse IL-10 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

In the mouse, Interleukin 10 (IL10), first characterized as a Cytokine Synthesis Inhibitory Factor (CSIF), is a 160 amino acid protein noncovalently associated in a homodimer. In humans, IL10 is produced by various T cell populations, monocytes, macrophages, and other cell types. IL10 activity is mediated by the heteromeric IL10 receptor (IL10R). IL10 inhibits interferon-? synthesis in Th1 cells and monocytes, and inhibits IL12 production by monocytes. It also impairs the proliferation and cytokine synthesis of CD4+ T cells, without having a direct inhibitory affect on CD8+ T cells. On the other hand, IL10 has a stimulatory influence on B cells. In general, its main biological functions are to limit and terminate the inflammatory responses, block proinflammatory cytokine secretion, and regulate the differentiation and proliferation of several immune cells.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.