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AlphaLISA Bovine IL-13 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for bovine IL-13 enables the quantitative determination of bovine interleukin-13 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL557HV
Unit Size: 100 Assay Points
List price: USD 680.80
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USD 680.80
USD 680.80 /each
Part number: AL557C
Unit Size: 500 Assay Points
List price: USD 2,256.00
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USD 0.00
USD 2,256.00 /each
Part number: AL557F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
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USD 0.00
USD 14,900.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interleukin 13 (IL13) is secreted by many cell types, especially T helper type 2 (Th2) cells. IL-13 is functionally similar to the IL4 and an important mediator of allergic airway-inflammatory diseases including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion. IL13 is known to play an important role in early pregnancy, vaccination, bovine respiratory syndrome. Levels of IL13 are associated with airway inflammation in asthma and other airway diseases. This kit is designed to detect IL13 in serum, plasma, and cell culture supernatants.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
IL13
Target Class
Biomarkers
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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Application Note
Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits

Leptospirosis is an infectious disease caused by Leptospira bacteria, which can spread across different species of mammals...

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