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AlphaLISA Human ICAM-1 Detection Kit, 500 Assay Points

The AlphaLISA® Human Intercellular Adhesion Molecule-1 (ICAM-1) Detection Kit is designed for detection and quantitation of human ICAM-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of ICAM-1. The analyte in this kit consists of the extracellular domain of human ICAM-1.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL282C
Unit Size: 500 Assay Points
Part Number: AL282F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Intercellular Adhesion Molecule-1 (ICAM-1) is a dimeric transmembrane glycoprotein with a molecular weight of 80 - 114 kDa. ICAM-1 is a member of the immunoglobulin family and can be expressed on non-hematopoietic cells, such as vascular endothelial cells. It is involved in the transmigration of leucocytes to sites of inflammation. A soluble form of ICAM-1 (sICAM-1) has been identified in serum and cerebrospinal fluid and it contains the five extracellular Ig-domains of ICAM-1. sICAM-1 is an inflammatory marker that has been associated with several common diseases such as diabetes, heart disease, stroke, and malaria. High levels of sICAM-1 have been found in serum from patients with cardiovascular disease, cancer, HIV-1, and autoimmune diseases. sICAM-1 is able to competitively bind the ligands of membrane-bound ICAM-1, such as LFA-1, Mac-1, and human rhinovirus.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Cardiovascular
    Unit Size
    500 Assay Points