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AlphaLISA Human COL1A1 Detection Kit, 100 Assay Points

The AlphaLISA® Human COL1A1 Detection Kit is designed for detection and quantitation of human COL1A1 in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL371C
Unit Size: 500 assay points
Part Number: AL371HV
Unit Size: 100 Assay Points
Part Number: AL371F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Collagen, type I, alpha 1 (COL1A1) is known as alpha-1 type I collagen that is produced by COL1A1 gene in the connective tissues. Upon synthesis as the pro-alpha1(I) chains, two pro-alpha1(I) chains and one a pro-alpha2(I) chain combine to make up rope-like procollagen molecules that are ultimately converted into Type I collagen by enzymes outside the cells. Type I collagen is well known to maintain and support connective tissues such as cartilage, bone, tendon, and skin. Changes of COL1A1 levels in cells, connective tissues, and in biological fluids are associated with diseases like Ehlers-Danlos syndrome, osteogenesis imperfecta, and osteoporosis.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Automation Compatible
    Yes
    Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target
    COL1A1
    Assay Target Class
    Biomarker
    Collagen
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Oncology
    Brand
    AlphaLISA
    Unit Size
    100 Assay Points