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AlphaLISA Human α1 Anti-Trypsin (α1AT) Detection Kit, 100 Assay Points

The AlphaLISA® Human A1AT Detection Kit is designed for detection and quantitation of human Alpha-1 Anti-Trypsin (A1AT) in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL344HV
Unit Size: 100 Assay Points
List price: USD 986.06
Your price:
USD 986.06
USD 986.06 /each
Part number: AL344C
Unit Size: 500 assay points
List price: USD 2,323.00
Your price:
USD 0.00
USD 2,323.00 /each
Part number: AL344F
Unit Size: 5,000 Assay Points
List price: USD 15,320.00
Your price:
USD 0.00
USD 15,320.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha-1 Antitrypsin (A1AT) is a 52 KD protein. The serpin superfamily includes the protease inhibitors alpha-1 antitrypsin and antithrombin, which are widely studied due to their involvement in the blood coagulation and inflammation. A deficiency in antitrypsin causes emphysema ((Chronic obstructive pulmonary disease (COPD), also known as chronic obstructive lung disease (COLD), and chronic obstructive airway disease (COAD)). Circulating levels of A1AT is an excellent indicator of patients with inflammatory diseases. The present kit detects hA1AT in human serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Quantity in a Package Amount
1.0 Units
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
100 Assay Points

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