Kelch-like ECH-associated protein 1 (KEAP1) is an adaptor subunit of Cullin 3-based E3 ubiquitin ligase involved in oxidative stress response. By promoting UPS mediated degradation, KEAP1 controls the expression level of key proteins such as NRF2, a transcription factor controlling the expression of antioxidant proteins. Sustained activation of NRF2 has been associated to cancers resistance, and its inhibition has been shown to dampen drugs resistance. Targeted protein degradation (TPD) uses small molecules to recruit E3 ubiquitin ligases into the proximity of the targeted protein of interest, promoting its ubiquitination-dependent degradation. Exploiting KEAP1 inhibitors for the development of bifunctional Proteolysis-targeting chimeras (PROTACs) is expected to expand the toolbox of E3 ligases to removing undesired proteins (e.g., NRF2) involved in various diseases such as cancers, neurodegenerative diseases, and metabolic disorders.

Formats

• Our 500 assay point kit allows you to run 500 wells in a 384-well format, using a 20 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in a 384-well format, using a 20 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Small sample volume

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).