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AlphaLISA Human FABP3 Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human fatty acid binding protein 3 (FABP3) enables the quantitative determination of human FABP3 in buffer, cell cultrue media, and serum using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3122HV
Unit Size: 100 Assay Points
Part Number: AL3122F
Unit Size: 5,000 Assay Points
Part Number: AL3122C
Unit Size: 500 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    AlphaLISA features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Fatty Acid-Binding Protein (FABP3) is also known as Heart-type FABP (H-FABP). FABP3 is a low molecular weight (15 kDa) protein located in the cytosol of the myocardium. During myocardial infarction, FABP3 is released into the blood where it circulates. Levels of FABP3 in the blood can be used as a marker for Acute Myocardial Infarction (AMI). Together with Troponin, FABP3 levels can be used to identify acute coronary syndrome in patients exhibiting angina.

  • Automation Compatible
    Yes
    Assay Points
    5000
    Assay Target
    FABP3
    Assay Target Class
    Protein
    Detection Method
    Alpha
    Therapeutic Area
    Cardiovascular
    Brand
    AlphaLISA
    Unit Size
    5,000 Assay Points