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AlphaLISA Human EGFR Detection Kit, 100 Assay Points

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL340HV
Unit Size: 100 Assay Points
List price: USD 1,094.00
Your price:
USD 1,094.00
USD 1,094.00 /each
Part number: AL340C
Unit Size: 500 assay points
List price: USD 2,393.00
Your price:
USD 0.00
USD 2,393.00 /each
Part number: AL340F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price:
USD 0.00
USD 15,950.00 /each

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Epidermal Growth Factor Receptor (EGFR, Her1, ErbB1) is a 134 kDa cell surface receptor apart of a four member subfamily of receptor tyrosine kinases (erbB1, erbB2, erbB3, and erbB4). EGFR binds to a family of proteins called the epidermal growth factors, which upon association induces dimerization of the receptor and initiates signal transduction for the promotion of cell survival and growth. Mutations that cause EGFR overexpression have been linked with many different types of cancer such as lung cancer or gastrointestinal tract cancers. Measuring the EGFR levels is vital for the detection of these types of cancers and is also an important target for therapeutic intervention. The AlphaLISA kit presented here has been designed for the detection of EGFR in cell culture media and serum.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Biomarker
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
100 Assay Points

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