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AlphaLISA Human CTLA-4 Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) enables the quantitative determination of human CTLA-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3050HV
Unit Size: 100 Assay Points
Part Number: AL3050C
Unit Size: 500 Assay Points
Part Number: AL3050F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Cytotoxic T-lymphocyte-associated protein 4 (CTLA4 or CTLA-4) is known as CD152 (cluster of differentiation 152). CTLA-4, as a protein receptor, is a critical biomarker for immune checkpoints. It is constitutively expressed in regulatory T cells (such as suppressor T cells) and the expression is upregulated in conventional T cells after activation. CTLA-4 acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. Thus, CTLA-4 is associated with numerous disease conditions such as insulin-dependent diabetes mellitus, systemic lupus erythematosus and other autoimmune diseases and cancers. Soluble CTLA-4 levels in biofluids have become a biomarker for determining the health status in patients. This kit is designed to detect and quantify the levels of CTLA-4 in cell culture supernatant, serum, and cell lysates.

  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target Class
    Biomarker
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Oncology
    Unit Size
    100 Assay Points

Resources

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Application Note
Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te

This study uses AlphaLISA kits to analyze the various parameters involved in the detection and quantification of membrane-based...

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