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AlphaLISA Human C-Reactive Protein Detection Kit, 5,000 Assay Points

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL233F
Unit Size: 5,000 Assay Points
Part Number: AL233C
Unit Size: 500 Assay Points
  • Formats:

    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    C-reactive Protein (CRP) is an acute-phase serum protein (206 amino acids), present in homopentameric form, that plays a role in the first line of host defense. In response to infection, inflammation, or tissue damage, IL6, IL1, and glucocorticoids induce the production of CRP in the liver. CRP exhibits Ca2+-dependent binding to ligands such as phosphorylcholine, a constituent of many bacterial and fungal cell walls, or the membrane of injured cells. It also binds to nuclear components of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans are associated with an increased risk of cardiovascular diseases. Its mechanism is not yet completely understood.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target
    CRP
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Inflammation
    Unit Size
    5,000 Assay Points