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AlphaLISA SARS-CoV2 ACE2/S1 Detection Kit, 500 Assay Points

The AlphaLISA™ SARS-CoV2 ACE2/S1 Binding Kit is a protein-protein interaction assay for ACE2-Spike inhibitor selection in biochemical format using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 5 µL

The AlphaLISA™ SARS-CoV2 ACE2/S1 Binding Kit is a protein-protein interaction assay for ACE2-Spike inhibitor selection in biochemical format using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
AL3146C
List Price
USD 3,338.00
Unit Size: 5,000 Assay Points
Part #:
AL3146F
List Price
USD 16,470.00

Overview

Angiotensin-converting enzyme 2 (ACE2) is a human cell surface receptor that has been identified as the main point of entry for SARS-CoV-2 virions, which match and bind ACE2 with their own viral Spike protein S1 subunits. This interaction is therefore a key target in COVID-19 therapeutic research, as its inhibitors may yield a protective effect by preventing SARS-CoV-2 entry into cells. For this reason, protein-protein interaction assays suitable for ACE2-Spike inhibitor screening are relevant to current research.

Formats:
  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).
Features:
  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume

The AlphaLISA detection of SARS-CoV-2 S1/ACE2 binding uses acceptor beads coupled to human ACE2 protein and Streptavidin-coated donor beads to capture the biotinylated S1-protein. Donor beads and acceptor beads come into proximity through S1 binding to ACE2. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp emission at 615 nm (Figure 1).This assay can facilitate the screening of inhibitors of S1/ACE2 binding. A competitor of the interaction will disrupt the S1/ACE2 binding leading to a decrease of the AlphaLISA Signal.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
SARS-CoV2 ACE2/S1
Target Class
Binding Assay
Technology
Alpha
Therapeutic Area
Virology
Unit Size
500 Assay Points

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