Pre-formulated buffer that can be used in histone H3 peptide-based epigenetic assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer prevents the non-specific binding of the highly-charged histone H3 (1-21) peptide to Donor and Acceptor beads in AlphaLISA epigenetic assays. Validated with Acceptor beads anti-H3K9ac (AL114), anti-H3K4me1-2 (AL116) and anti-H3K9me2 (AL117). It is used at the detection step for the dilution of Acceptor and Donor beads, not for the enzymatic reaction step. This buffer is not recommended for assays using full length histone proteins or nucleosomes.
Formulation: proprietary
Application |
Epigenetics
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
1.0 each
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
10 mL
|
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