Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-Nrf2 (Ser40) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-Nrf2 (Ser40) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-Nrf2 (Ser40) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were then treated with 10 μM MG132 and 2 μM PMA for 18 hours and lysed in 3 mL of SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Assay Target |
Nrf2
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
|
Unit Size |
1 Vial
|
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