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AlphaLISA Surefire Ultra Total NFκB Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra total NFκB assay. This control is already provided in the AlphaLISA SureFire Ultra total NFκB assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TNFKB-A-L
Part Number: ALSU-TNFKB-A-L
  • Positive Control Lysate is prepared from HeLa cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 50 ng/mL TNFα plus 20 ng/mL Calyculin A for 10 minutes and lysed in 10 mL of 1X SureFire Ultra Lysis buffer.

  • Application
    Kinase
    Protein Analysis & Detection
    Assay Target Class
    Protein
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Metabolic
    Unit Size
    1 vial

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...