AlphaLISA SureFire Ultra Human and Mouse Total IRF5 Detection Kit, Control Lysate

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AlphaLISA SureFire Ultra Human and Mouse Total IRF5 Detection Kit, Control Lysate

COAs

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Total IRF5 assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Total IRF5 assay kit, but can also be ordered separately.

Product information

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

COAs

Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Total IRF5 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 300K/mL, and incubated for 48 hours in T175 flasks in 10% FBS containing medium. Cells were harvested and resuspended at 8 x 10⁶ cells/mL and treated with 100 nM Calyculin A for 3 hours. Cells were then washed in HBSS + 0.1% BSA and lysed in Lysis Buffer at a final cell density of 8 x 10⁶ cells/mL.

Specifications

Other Specifications

Assay Target	IRF5
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Method	Alpha
Predicted Species Reactivity	Human Mouse
Shipping Conditions	Shipped in Blue Ice
Therapeutic Area	Inflammation Oncology
Unit Size	1 vial