Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-Aurora A (Thr288) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-Aurora A (Thr288) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-Aurora A (Thr288) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in10% FBS containing medium, then treated with 500 nM Nocodazole and 1 μM Okadaic Acid for 2 hours at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Assay Target |
Aurora A
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
|
Therapeutic Area |
Oncology
|
Unit Size |
1 Vial
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.