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AlphaLISA Surefire Ultra Human, Mouse, and Rat Aggregated α-Synuclein Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ alpha synuclein aggregation assay kit can be used to measure levels of aggregated alpha-synuclein in cellular lysates using no-wash Alpha technology. The antibodies recognize distinct epitopes specific to the Aggregated form of α-Synuclein.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-AASYN-A500
Unit Size: 500 Assay Points
Part Number: ALSU-AASYN-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-AASYN-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-AASYN-A-HV
Unit Size: 100 Assay Points
  • α-Synuclein is a neuronal protein encoded by the SNCA gene that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. α-Synuclein is a prominent component of intracellular fibrillary aggregates in the brains of patients suffering from synucleinopathies. This protein represents a key molecular hallmark for Parkinson’s disease, dementia with Lewy bodies, or multiple system atrophy. The extensive phosphorylation of α-Synuclein on Ser129 is also associated with a pathological event.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA®SureFire®Ultra™ kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Detecting alpha synuclein aggregation
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
    • Replacing an alpha synuclein ELISA kit
  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Therapeutic Area
    Central Nervous System
    Unit Size
    100 Assay Points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...