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PhenoVue Cell Painting Kits

Cell Painting is a powerful and new phenotypic high-content screening approach which combines cell and computational biology to unravel cells responses when subjected to perturbagens, such as chemical compounds, or gene expression modulators.

In this process, cells are “painted” by labeling various cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters to better understand the effects of chemical compounds, drugs, genes, or other test articles. Cell compartments and organelles are simultaneously tagged with six fluorescent probes, followed by acquisition and analysis of images. The six probes target specific cell compartments to determine protein expression or signaling pathways, identify organelles and their function, or identify whole-cell morphology.

It is recommended that researchers follow the protocols described in the Bray et al. (2016) publication or published by the JUMP consortium. Revvity PhenoVue cell painting kits (two versions available) have been designed to match the experimental conditions described and comprise validated, preoptimized fluorescent bioprobes to streamline workflows, saving you time and cost. Each kit contains all the necessary cell painting reagents (stains/fluors and diluent) as well as a step-by-step protocol to maximize your cell painting assay efficiency. The protocols are for use with 384-well microplates but can easily be adapted for use with 96-well microplates.

Proud to be supporting the JUMP-CP consortium

We are proud to be supporting the Joint Undertaking in Morphological Profiling-Cell Painting (JUMP-CP) consortium, providing our PhenoVue Cell Painting JUMP kits to the Consortium to create the world’s largest, public cell painting dataset. See our press release in the Resources tab for more details.

References:

  1.  Bray M, Singh S, Han H, Davis C, Borgeson B, Hartland C et al. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Nature Protocols. 2016;11(9):1757-1774.
  2.  JUMP-Cell Painting Consortium [Internet]. Jump-cellpainting. broadinstitute.org. [cited 01 December 2022]. Available from: https://jump-cellpainting.broadinstitute.org/. Preprint available at: https://www.biorxiv.org/content/10.1101/2022.07.13.499171v1

For research use only. Not for use in diagnostic procedures.

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PhenoVue Cell Painting JUMP Kit (for 10x & 100x wells)
USD 159.85 - 8,304.00
PhenoVue Cell Painting Kit
USD 150.51 - 8,408.00
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Whitepaper
AAV vectors: Pursuing a one-time therapy for ocular diseases

In the ground-breaking realm of gene therapy, adeno-associated virus (AAV) vectors are revolutionizing our approach to treating...

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Cell painting - a cellular imaging and machine learning approach to drug discovery

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Cell painting for phenotypic screening

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Cell painting: your questions answered

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Phenotypic analysis of CRISPR-Cas9 cell-cycle knockouts using cell painting

Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and...

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Product Info
PhenoVue Cell Painting JUMP Kit, 1 x 384-wells Product Information Sheet

Details of PhenoVue Cell Painting JUMP Kit, 1 x 384-wells.

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PhenoVue Cell Painting JUMP Kit, 10 x 384-wells Product Information Sheet

Details of PhenoVue Cell Painting JUMP Kit, 10 x 384-wells.

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PhenoVue Cell Painting Kit 1 x 384-Well Plate Product Information Sheet

This is a product information sheet for PhenoVue Cell Painting Kit 1 x 384-Well Plate.

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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PhenoVue Reagents & Imaging Microplates Product List
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Product Information Sheet for PhenoVue Cell Painting Kit 10 x 384-Well Plates
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