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Cellular Internalization Kits and Reagents

Internalization and trafficking to lysosomes are critical processes for both antibody-drug conjugates (ADCs) and G protein-coupled receptor (GPCR) therapeutics. For ADCs, internalization enables payload release in acidic endo-lysosomal compartments following target binding, while GPCR internalization regulates receptor availability at the cell surface, controlling desensitization and the duration of receptor-mediated signaling. Effective methods to quantify internalization in live cells are essential for both ADC development and GPCR characterization.

Revvity provides tools for studying internalization using both plate-reader based assays and high-content imaging. These approaches are complementary:

pHSense™ probes are plate reader-compatible reagents with time-resolved fluorescence (TRF) readout. These probes empower researchers with a scalable, sensitive, and easy-to-implement solution.

PhenoVue™ reagents for internalization studies facilitate real-time visualization of antibody trafficking into acidic cellular lysosomes with clarity and reliability.


For research use only. Not for use in diagnostic procedures.

pHSense schematic

Hear from our customers

"We use pHSense and PhenoVue as complementary tools at different stages of our ADC development workflow, and the combination is powerful. We start with pHSense screening to quickly identify promising antibody candidates from our panel. It's fast, easy, and perfect for high-throughput evaluation. Then we follow up with PhenoVue imaging experiments on our top candidates to gain deeper mechanistic insight into trafficking pathways and intracellular localization. This two-stage approach gives us both the speed we need for screening and the detailed understanding required for confident candidate selection. It's a workflow that actually makes sense."

Frédéric Delobel
Ridgeline

"With pHSense, my experience was very positive. The assay itself was straightforward to set up and run, and I was able to detect a clear, specific signal within just 20 minutes, compared to the 3 hours needed with other probes. The signal-to-noise ratio was greatly improved in my tests, allowing for further flexibility and optimization options. The support provided by the Revvity team was very helpful. Overall, I would recommend pHSense for groups investigating antibody internalisation in adherent cell systems."

Dimitri Sideris
The Discovery Centre (DISC) - AstraZeneca, UK

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