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Thymidine, [Methyl-3H]-, Specific Activity: 2 Ci/mmol, 5 mCi

Thymidine, tritiated on the methyl group. This product is supplied steri-packaged in an aqueous solution, and is typically used in DNA cell proliferation assays.

For research use only. Not for use in diagnostic procedures

Product Variants
Part number: NET027A250UC
Unit Size: 250 µCi
List price: USD 727.54
Your price:
USD 0.00
USD 727.54 /each
Part number: NET027A001MC
Unit Size: 1 mCi
List price: USD 1,305.00
Your price:
USD 0.00
USD 1,305.00 /each
Part number: NET027A005MC
Unit Size: 5 mCi
List price: USD 3,145.00
Your price:
USD 3,145.00
USD 3,145.00 /each


5 mCi quantity of [Methyl-3H]-Thymidine is available for your research. Application of [3H]Thymidine can be found in: carboxy-terminal PTH fragments stimulating incorporation in vascular endothelial cells, quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity in juvenile myelomonocytic leukemia (JMML), influence of parasympathetic non-adrenergic, non-cholinergic mechanisms on uptake (rat) in response to mastication, measurement of dendritic cell induced T cell response by intracellular ATP levels and uptake in human immunology, etc.

Special Information: If aqueous solutions of thymidine are stored frozen (-20°C), care should be taken to protect the material from U.V. light during the thawing process to prevent possible dimerization.

When [methyl-3H]thymidine is incorporated into cellular DNA and undergoes radiolytic decomposition, [3H]5-hydroxymethyl-2''-deoxyuridine (HMdU) is formed.* The formation of HMdU through transmutation of [methyl-3H]thymidine occurs at the rate of B-decay, which is 0.017% per day. The transmutation product is not volatile.


  • S. Apelgot and B. Ekert, J. Chem. Phys. 60, 505 (1963).
  • K. Fink, R. E. Cline, and R. M. Fink, Anal. Chem., 35, 389 (1963).
  • Specifications and Criteria for Biochemical Compounds, Third Edition (ISBN 0-309-01917-6), National Academy of Sciences - National Research Council, Washington, D.C. 1972.
  • G. W. Teebor, K. Frenkel, and M. S. Goldstein, Proc. Natl. Acad. Sci. USA 81, 318 (1984).
  • H. Tono, S.S. Cohen, J. Biol. Chem. 237, 1271 (1962).
  • M. Nemer, J. Biol. Chem. 237, 143 (1962).


Molecular Weight
Molecular Biology
NEN Radiochemicals
Detection Method
Label Position
Specifically Labeled
Special Ordering Information
This is a radioactive product - shipping address must have a license to receive radioactive materials.
Unit Size
5 mCi


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