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Streptavidin YOX SPA Imaging Beads 500 mg

Streptavidin-coated SPA beads, for capturing biotinylated molecules in proximity-based scintillation assays.

For research use only. Not for use in diagnostic procedures
Part Number: RPNQ0271
Part Number: RPNQ0271
  • Streptavidin-coated yttrium oxide (YOx) SPA beads, for capturing biotinylated molecules in proximity-based scintillation assays. 500 mg quantity.

    SPA Imaging beads are microspheres containing scintillant which emit light in the red region of the visible spectrum. As a result, these beads are ideally suited to use with a CCD imager.

    Two types of core SPA Imaging bead are available - yttrium oxide (YOx) and Polystyrene (PS). A number of biological coatings also exist for each core bead type to enable receptor binding, enzyme, molecular interaction and radioimmunoassays to be investigated.

    Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

  • Application
    Drug Discovery & Development
    Automation Compatible
    No
    Bead Type or Core Bead Type
    Yttrium Oxide (YOx)
    Brand
    SPA Imaging Beads
    Coating Treatment
    Streptavidin
    Detection Method
    Radiometric
    Shipping Conditions
    Shipped Ambient
    Unit Size
    500 mg