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DELFIA Streptavidin-coated yellow plate, 96-well, 10 plates

96-well yellow microplates coated with streptavidin, for biotinylated biomolecules for use in DELFIA time-resolved fluorescence assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part number: AAAND-0005
List price: USD 680.55
Your price:
USD 680.55
USD 680.55 /each

Overview

Streptavidin-coated DELFIA® plates bind all biotinylated biomolecules; e.g. peptides, proteins, antibodies, and haptens. The biotin binding capacity is 10 pmol/well in 200 µL volume. DELFIA Yellow plates have an exceptionally low fluorescence background.

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Color
Yellow
Well Format
Application
Research
Automation Compatible
Yes
Brand
DELFIA
Coating Treatment
Streptavidin
Detection Method
Time-Resolved Fluorescence (TRF)
Format
Microplates
Material
Polystyrene
Shipping Conditions
Shipped Ambient
Sterility
Non-Sterile
Unit Size
Pack of 10

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
Qualification of a DELFIA Assay for the Detection of Anti-mouse IgG Antibodies in Human Serum

We report here the development of a Dissociation Enhanced Lanthanide Fluorescence Immunoassay (DELFIA) for the detection of ADA in...

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