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Copper HIS-Tag PVT SPA Beads, 250 mg

SPA (scintillation proximity assay) beads designed for the capture of His-tagged proteins and peptides.

For research use only. Not for use in diagnostic procedures
Part Number: RPNQ0095
Part Number: RPNQ0095
  • Copper HIS-Tag Polyvinyltoluene (PVT) SPA (scintillation proximity assay) beads designed for the capture of His-tagged proteins and peptides. 250 mg quantity.

    SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.

    Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.

    Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

  • Automation Compatible
    Yes
    Application
    Drug Discovery & Development
    Bead Type or Core Bead Type
    PVT
    Coating Treatment
    Copper chelate
    Copper (HIS-tag)
    Detection Method
    Radiometric
    Brand
    SPA Scintillation Beads
    Unit Size
    250 mg