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AlphaLISA Human Vimentin Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human vimentin enables the quantitative determination of human vimentin in serum, buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL372HV
Unit Size: 100 Assay Points
Part Number: AL372C
Unit Size: 500 Assay Points
Part Number: AL372F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Vimentin is one of the components of cell intermediate filaments. It is a member of the class III filament proteins, and is the main cytoskeletal component of mesenchymal cells. It is also present in other non-epithelial cells It is widely used as a marker for the presence and growth of sarcoma cancers. Modified forms of vimentin (such as citrunilated forms) are also markers of arthritis and other inflammations. This kit allows for the detection of vimentin in cell culture media and cell extracts.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Biomarker
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Therapeutic Area
    Oncology
    Unit Size
    5,000 Assay Points