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AlphaLISA Human TSH Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3005HV
Unit Size: 100 Assay Points
Part Number: AL3005C
Unit Size: 500 Assay Points
Part Number: AL3005F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    TSH (thyroid stimulating hormone), also known as thyrotropin, is a member of the cysteine knot growth factor superfamily. It is a heterodimer consisting of a 15 kDa unique TSH beta subunit and a 14 kDa alpha subunit, common glycoprotein hormone alpha that is shared with Luteinizing Hormone, Follicle Stimulating Hormone and Human Chorionic Gonadotropin. Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH. TSH binds to thyroid TSH receptors to stimulate production of thyroxine (T4). In tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which stimulates the metabolism of almost every tissue in the body and completes a feedback loop by inhibiting TSH production. Serum TSH measurement is a crucial tool for the diagnosis of thyroid disorders. Increased serum TSH is an early and sensitive indicator of decreased thyroid reserve and overt primary hypothyroidism. Decreased TSH level is an indicator of TSH-independent hyperthyroidism (Graves' disease). For recombinant hTSH, conversion for IU is 1 µg of protein equals 7.9 x 10-3 IU.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    Assay Target Class
    Automation Compatible
    Detection Method
    Experimental Type
    In vitro
    Unit Size
    500 Assay Points