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AlphaLISA Human PDGF-BB/PDGFRβ Binding Kit, 500 Assay Points

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 5 µL

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
AL3065C
List Price
USD 2,751.00
Unit Size: 5,000 Assay Points
Part #:
AL3065F
List Price
USD 22,240.00

Overview

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaLISA technology allows detecting the binding of target proteins in a highly sensitive, quantitative, reproducible and user-friendly mode. In this AlphaLISA assay, a biotinylated PDGF-BB binds to the Streptavidin-coated Alpha Donor beads, while His tagged PDGFRβ is captured by Anti-His AlphaLISA Acceptor beads. When PDGF-BB binding to PDGFRβ happens, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division. PDGF is a dimeric glycoprotein that can be composed of two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). The dimeric isoforms are differentially expressed in various cell types and their effects are mediated through two cell surface tyrosine kinase receptors, platelet-derived growth factor receptors, termed alpha (PDGFRβ) and beta (PDGFRβ). Differences exist in isoform binding to each receptor. PDGF and PDGFR are involved in human cancer development and progression through autocrine stimulation of tumor cell growth. Blocking PDGF and PDGFR binding has been considered as a potential target for antitumor and antiangiogenic therapy.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
PDGF-BB, PDGFR
Target Class
Binding Assay
Target Species
Human
Technology
Alpha
Therapeutic Area
Immuno-oncology
Unit Size
500 Assay Points

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