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AlphaLISA Human KIM-1 Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human Kidney Injury Molecule 1 (KIM-1) enables the quantitative determination of human KIM-1 in buffered solution, urine, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3075HV
Unit Size: 100 Assay Points
Part Number: AL3075C
Unit Size: 500 Assay Points
Part Number: AL3075F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Kidney Injury Molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor1 (HAVcr-1) and T-cell immunoglobulin and mucin domain 1 (TIM-1), is a type 1 transmembrane protein containing mucin and immunoglobulin domains. KIM-1 is widely expressed with the highest levels in kidney and testis. KIM-1 serves as a biomarker of renal damage, specifically proximal tubular injury. Patients with renal carcinoma or acute tubular necrosis, for example, experience a drastic increase of KIM-1 in their serum or urine. This detection kit is designed to quantify KIM-1 in urine, serum and cell culture media.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Therapeutic Area
    Metabolic
    Unit Size
    5,000 Assay Points