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AlphaLISA Human Heme oxygenase 2 Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3027HV
Unit Size: 100 Assay Points
List price: USD 680.80
Your price:
USD 680.80
USD 680.80 /each
Part number: AL3027C
Unit Size: 500 Assay Points
List price: USD 2,256.00
Your price:
USD 0.00
USD 2,256.00 /each
Part number: AL3027F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
Your price:
USD 0.00
USD 14,900.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Heme oxygenases are enzymes that are involved in the degradation of heme. They cleave the heme ring to create billiverdin, which then becomes bilirubin due to the action of bilirubin reductase. This cascade allows for the removal of heme in damaged red blood cells as well as heme bound irreversibly to molecules such as carbon monoxide. Heme oxygenases have been characterized as markers for oxidative stress, metabolic disorders and inflammation. Heme oxygenase 2 is present mainly in the brain. Its role appears to be protective against oxidative stress. Production of carbon monoxide from the cleavage of the heme ring seems to play a protective role against malaria by poisoning the hemoglobin essential for the parasite growth.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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